Advances in Heart Failure |
From the Heart Failure Unit, Cardiopulmonary Laboratory, Department of Medicine and Surgery, San Paolo Hospital, University of Milano, Italy.
Correspondence to Marco Guazzi, MD, PhD, FACC, Heart Failure Unit, Cardiopulmonary Laboratory, University of Milano, San Paolo Hospital, Via A. di Rudinì, 8, 20142 Milano, Italy. E-mail marco.guazzi{at}unimi.it
Received June 25, 2008; accepted September 16, 2008.
Key Words: heart failure pharmacology
| Introduction |
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| Phosphodiesterases |
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| PDE5 Inhibitors |
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The onset of action of sildenafil is rapid and plasma half-life is of 4 hours.11–13 Empirical testing shows that the duration of action of sildenafil may reach 12 hours.14 The results of numerous studies consistently suggest that more commonly used drugs do not disturb the pharmacokinetics of sildenafil, and that the compound is well tolerated without interaction with the physiological effects of most drugs.
| PDE5 Inhibition in Heart Failure: Rationales and Evidences |
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| Lung |
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Studies performed by Guazzi et al24 and by Lewis et al25 in patients with CHF and secondary pulmonary hypertension, have shown that acute oral sildenafil (50 mg) lowers the pulmonary vascular pressure and resistance, without significantly affecting the systemic arterial pressure and the wedge pulmonary pressure. The response of cardiac output is variable and in part related to the severity of the disease. The ability to reduce pulmonary vascular resistance at rest is also evident during exercise.25 Recently, the same authors have reported that the PDE5 inhibitor maintains efficacy during long-term administration.26,27
An additional effect of sildenafil on the lung24 is an improvement of the diffusion capacity for carbon monoxide by more than 10% in patients with CHF, through a selective increase of the alveolar-capillary membrane gas conductance (DM), without affecting the pulmonary capillary volume of blood available for gas exchange (DM and pulmonary capillary volume of blood are the 2 components of diffusion capacity for carbon monoxide). This proves that PDE5 inhibition can affect one of the lung function abnormalities produced by CHF, it also suggests that a defective NO/cGMP pathway may have a putative role in the excessive resistance to gas transfer across the blood gas barrier. On a clinical setting, a debated issue is whether impairment in gas transport at the alveolar-capillary junction is significantly involved in exercise intolerance in CHF.28–30 The correlations of baseline diffusion capacity for carbon monoxide with peak exercise oxygen uptake,30 and of DM with the ventilatory reaction to exercise in relation to the carbon dioxide output (VE/VCO2)31 and with the arterial oxygen saturation at peak exercise,32 support a lung diffusion limitation as one of the mediators of exercise impairment in heart failure. It is clinically significant, therefore, that sildenafil is effective on alveolar-capillary conductance and that the improvement of DM with sildenafil is tightly related with that of VE/VCO2.24
| Heart |
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Despite early documentation of PDE5 gene expression in the myocardium,35 enzyme activity is rather low compared with lung and has been currently considered as physiologically not significant.36 More recent studies, however, have provided evidence of PDE5 expression in isolated myocytes, of myocyte physiological effects of sildenafil and tadalafil37 and of whole heart effects of PDE5 inhibitors.37,38 PDE5 inhibition exerts little influence on cardiac function in the baseline, but inhibits acute β-adrenergic stimulation.37,39 The adrenergic effect of sildenafil is lacking in myocytes deficient in endothelial NO synthase or with NOS inhibited by NG-nitro-L-arginine methyl ester, suggesting a main role of NO-generated cGMP.40 In a number of chronic cardiovascular diseases, cGMP increases often in response to sustained release of natriuretic peptides, and PDE5 is upregulated in pulmonary hypertension,41 RV hypertrophy,42 congestive heart failure.43 A correlate of PDE5 upregulation is that effects of its counteraction can be emphasized. In the hypertrophied human right ventricle, PDE5 is highly expressed and its inhibition improves contractility,42 thus showing that this upregulation is physiologically significant. Ockaili et al44 first demonstrated that sildenafil induces acute and delayed protective effects against ischemia-reperfusion injury, which are mediated by opening of mitochondrial potassium adenosine triphosphate (KATP) channels: the infarct size in treated rabbits was reduced from the value in control animals by 68%, during the acute phase, and by 41% during the delayed phase. Salloum et al45 have shown that endothelial NO synthase and inducible NO synthase are prominent players in protection mediated by sildenafil. The same group46 has reported similar results with vardenafil in rabbits. Elrod et al,47 however, have then observed in a murine model that reduction of myocardial ischemia/reperfusion injury by sildenafil may be independent of both endothelial NO synthase and inducible NO synthase, because protection is also seen in null animals and at doses of sildenafil that do not alter cGMP levels. These authors have also reported that sildenafil is not effective in diabetic mice subjected to myocardial ischemia/reperfusion injury, and have suggested that delineation of the mechanisms involved in sildenafil-mediated cardioprotection requires further research, mainly for translation to the clinical setting. Recently, Salloum et al48 have demonstrated that acute and prolonged treatment with sildenafil during myocardial infarction is associated with myocardial salvage from necrosis during the first 24 hours, reduction of apoptosis at 7 and 28 days, prevention of severe cardiac remodeling and heart failure, and improved survival. In this study, PDE5 inhibition has been prospected as a promising means for heart failure prevention in acute postmyocardial infarction patients.
Landmark experiments by Takimoto et al49 performed in mice exposed to sustained pressure overload, have documented that chronic PDE5 inhibition can prevent and reverse cardiac and myocite hypertrophy and interstitial fibrosis. Mechanistic insights provided by this study suggest that sildenafil deactivates multiple hypertrophy signaling pathways triggered by pressure overload (the calcineurin/fatty acid transport protein, extracellular signal regulated kinase/mitogen-activated protein kinase, and Akt). This modulatory activity was not observed in cases of hypertrophy induced in vitro, suggesting upstream targeting of these pathways. A simplified diagram depicting the potential molecular pathways involved in the antihypertrophic effects of PDE5 inhibition is reported in Figure 2.
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Other investigators have reported that PDE5 inhibition suppresses hypertrophy induced by catecholamine stimulation.51 Reversal of hypertrophy may stem from myocyte and, in intact animals, also from nonmyocyte effects of PDE5 inhibition. Nonmyocyte effects may include systemic, pulmonary and renal vascular changes, hormone and cytokine release from different cell types, myofibroblast, inflammatory responses, and antifibrotic activity.52 The molecular mechanisms, however, whereby PDE5 inhibitors produce antihypertrophic effects remain basically unclear.34
| Systemic and Pulmonary Hemodynamics and Endothelium |
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More than two thirds of patients with severe left ventricular systolic function impairment have secondary pulmonary pressure elevation and impaired RV performance.56 These are significant determinants of functional status and prognosis, because RV dysfunction increases by more than 2-folds mortality compared with patients with similar left ventricular function and preserved RV performance. With the exception of the combination of isosorbide dinitrate and hydralazine,57 use of drugs with pulmonary vasodilatory properties, including endothelin receptor antagonists and prostacyclin analogues, has not been effective for the treatment of heart failure due to left ventricular systolic dysfunction.58,59 Thus, based on the high prevalence of pulmonary vasoconstriction and secondary pulmonary hypertension in heart failure, new strategies to reduce pulmonary vascular tone and impedance to RV ejection are quite desirable. Sildenafil is a selective pulmonary vasodilator24,25 that in CHF reduces secondary pulmonary pressure elevation, increases RV systolic function, possibly due to reduced afterload60 and improves contractility,42 and exercise capacity to an extent proportional to the baseline level of the pulmonary vascular resistance.27 Thus, PDE5 inhibition seems very attractive for patients in whom CHF selectively targets the pulmonary vascular tone.
More than one study suggest that sildenafil may reverse endothelial dysfunction in heart failure.61,62 In a report in which sildenafil was tested in patients with stable CHF, at the doses of 12.5, 25, and 50 mg, flow-mediated dilatation in the forearm circulation increased in a dose-dependent fashion and the lower effective dose was shown to be 25 mg.61 When combined with the ACE inhibitor ramipril, sildenafil produced an additional benefit on endothelial function in patients with heart failure.63 In a canine model of cardiac failure, the hemodynamic influences of sildenafil were similar to those of the B-type natriuretic peptide43 and potentiated the pulmonary pressure lowering effect. An additional reason why PDE5 inhibition may be beneficial in CHF is that resistance to natriuretic peptide in the syndrome is in part related to an increased PDE5 activity.
| Exercise Performance and Gas Exchange |
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Sildenafil has been shown, both in acute24,25 and long-term studies,26,27,66 to properly reduce breathlessness sensation and VE/VCO2 slope on exercise. This effect seems to depend on a combination of favorable mechanisms. First, a decrease of waste ventilation (ie, reduced dead space to tidal volume ratio) and a facilitated DM.24,26 In addition, it has been recently reported that sildenafil intake can produce an endothelium-mediated attenuation of the ergoreflex stimulus to hyperventilation and breathlessness.26,62
Tables 2 and 3
summarize the clinical studies investigating the effects of acute and chronic use of PDE5 inhibitors in CHF patients.
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| Safety and Tolerability |
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Thus far there are no published data on safety or efficacy of other PDE5 inhibitors, vardenafil and tadanafil, in patients with heart failure. Differences in pharmacokinetics and selectivity among PDE5 inhibitors in clinical use suggest that safety and efficacy should be evaluated for each single preparation.
| Conclusions and Perspectives |
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A number of theoretical backgrounds and progressively accumulating evidence support the usefulness of NO potentiating compounds in CHF. Reduction of impedance to RV ejection and increase of RV output is an innovative way of treatment of CHF and patients with predominant pulmonary vasoconstriction and pressure elevation may be those who mostly benefit from prescription of PDE5 inhibitors. This is one of the main topics that deserve definitive evidence from large-scale clinical trials.
Initial promising perspectives await definition of safety, tolerability and potential impact of PDE5 inhibition on morbidity and mortality across the wide spectrum of heart failure populations. The first multicenter trial examining the clinical use of PDE5 inhibitors in heart failure (RELAX) will test the efficacy of chronic sildenafil to treat heart failure patients without dilatation and preserved ejection fraction (referred to as diastolic heart failure). Affected patients are generally more elderly, are more frequently females, often have high blood pressure and left ventricular hypertrophy, and nearly 40% have pulmonary hypertension. The main end points of the trial will include metabolic exercise capacity, left ventricular mass and function changes.
| Acknowledgments |
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The study was supported by the Monzino Foundation and by the University FIRST research grants.
Disclosures
None.
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